矮秆基因在大豆育种中的研究进展

    Research Progress on Dwarf Genes in Soybean

    • 摘要: 大豆作为全球重要的粮油饲兼用作物, 其产量水平直接关系国家粮食安全与农业可持续发展。株高是大豆株型中的关键产量构成因子, 通过主茎节数与节间长度的协同调控, 显著影响单株荚数形成、种植密度优化、抗倒伏能力提升与产量潜力挖掘。尽管水稻、小麦等主粮作物已通过半矮秆品种选育成功实现产量飞跃, 并引发“绿色革命”, 但大豆单产水平却长期停滞不前, 这凸显出半矮秆基因挖掘及其分子机制解析在大豆育种应用中的紧迫性。该文系统综述大豆株高构成因子中主茎节数与节间长度的遗传基础及其分子调控网络。一方面全面总结大豆株型特异的茎生长习性控制基因及其所处的分子通路, 主要包括Dt1Dt2基因在茎顶端分生组织中的表达特征及其对有限型、亚有限型和无限型茎生长习性的调控作用, 并探讨不同茎生长习性与生态适应性的关联性; 在分子调控层面, 大豆生长点中形成以Dt1Dt2为核心的复杂调控网络, 整合多基因与多信号分子实现对大豆株高的调控。另一方面, 该文重点综述赤霉素、生长素及油菜素甾醇等关键植物激素信号通路在大豆株高调控方面的最新研究进展, 赤霉素途径的研究主要集中在生物合成、代谢调控及信号转导方面的分子机制, 油菜素甾醇则被发现通过调控株型结构显著提升大豆产量潜力。基于大豆独特的株型特性与基因组的复杂性, 该文进一步从分子设计育种角度展望大豆半矮秆育种与产量提升的潜在路径, 以期为大豆利用半矮秆基因进行遗传改良和种质创新提供一定的理论基础。

       

      Abstract: Soybean is an important crop that can be used as both food, oil and forage in the world, and its yield is directly related to national food security and the sustainable development of agriculture. Plant height is a key yield component in plant type, significantly affects the formation of pod numbers per plant, the optimization of planting density, the improvement of lodging resistance and the exploration of yield potential through the synergistic effect of the number of main stem nodes and the length of internodes. Although major food crops such as rice and wheat have achieved a leap in yield through the breeding of semi-dwarf varieties and triggered a "green revolution", the per-unit yield of soybean has remained stagnant for a long time. This highlights the urgency of mining the semi-dwarf genes and analyzing their molecular mechanisms in soybean breeding research. This review systematically reviews the genetic basis and molecular regulatory network of the main stem node number and internode length among the constituent factors of soybean plant height. On the one hand, comprehensively summarizes the stem growth habit control genes specific to soybean plant types and the molecular pathways they are located in, mainly including the expression characteristics of Dt1 and Dt2 genes in the apical meristem and their regulatory effects on the growth habits of determinate, semideterminate and indeterminate types, and explore the correlation between different stem growth habits and ecological adaptability; At the molecular regulatory level, a complex regulatory network centered on Dt1 and Dt2 genes is formed in the apical meristem of soybean, integrating multiple genes and multiple signaling molecules to regulate the plant height in soybean. On the other hand, the latest research progress on the regulation of soybean plant height by the key plant hormone signaling pathways such as gibberellin, auxin and brassinosterol was reviewed. The research on the gibberellin pathway mainly focuses on the molecular mechanisms of biosynthesis, metabolic regulation and signal transduction, while brassinosterol has been found to significantly increase yield potential through the regulation of plant structure. Based on the unique plant type characteristics and the complexity of the genome in soybean, the review further looks forward to the potential paths of semi-dwarf soybean breeding and yield increase from the perspective of molecular design breeding, with the aim of providing a certain theoretical basis for genetic improvement and germplasm innovation of soybean using semi-dwarf genes.