Abstract: Aflatoxin B₁ (AFB₁) is a highly carcinogenic fungal toxin produced by the metabolism of Aspergillus flavus, posing significant risks to human and animal health as well as agricultural economics. Given the extreme hazards of AFB₁, its prevention and control have become a critical research focus for ensuring food safety. This paper reviews methods and measures for reducing the toxicity of AFB₁ using physical, chemical, and biological technologies, while also explaining the degradation mechanisms of AFB₁ under different detoxification technologies. However, while each individual detoxification technology has its advantages, they also have certain limitations. Therefore, this paper proposes a strategy for combining multiple detoxification technologies. By integrating the advantages of various detoxification technologies, this approach can effectively overcome the limitations of individual technologies, significantly improve the efficiency of AFB₁ detoxification, reduce the toxicity of degradation products, and minimize the impact on grain quality and nutritional components, demonstrating significant innovation and practical value. Additionally, future research directions for aflatoxin detoxification technology are explored, providing new insights and solutions for AFB₁ detoxification technology.