Abstract:
Objective Grape anthracnose is one of the most serious and common grape diseases in China. The pathogens involve multiple species and subspecies of Colletotrichum gloeosporioides and C. acutatum complex. The growth and epidemic characteristics of different species of pathogens are significantly different. Clarifying the species of grape anthracnose pathogens in Zhejiang Province can provide a reference for the population distribution of grape anthracnose pathogens and the green prevention and control of diseases in China.
Method Grape anthracnose samples were collected, and pathogens were isolated from the diseased parts of fruits. The pathogen was purified by subculture, and the colony, spore morphology and appressorium morphology of the pathogen were observed. The phylogenetic tree was constructed by using the sequences of ITS, TUB2, GAPDH, ACT, CHS and CAL. The pathogenicity of the pathogen was determined by artificial inoculation, and the effects of different temperature, pH value, carbon source and nitrogen source on the growth of the pathogen were determined.
Result Six strains of pathogens with basically the same morphological characteristics were isolated from the diseased parts of grape fruits. Koch's test showed that the strain was the pathogen of the disease. Combined with the morphological characteristics of the pathogen and the phylogenetic analysis based on ITS, TUB2, GAPDH, ACT, CHS and CAL gene sequences, the pathogen was identified as C. aenigma. The biological characteristics of the pathogen were analyzed. The results showed that the optimum culture temperature and sporulation temperature of the pathogen were 28℃, the optimum growth pH was 6, and the optimum carbon and nitrogen sources were maltose and beef extract, respectively.
Conclusion In recent years, the pathogen of grape anthracnose in Ninghai County, Zhejiang Province is mainly C. aenigma. And its biological characteristis were clarified.
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