大白菜长链非编码RNA研究进展

    Progress in Research on the Long Non-coding RNAs in Chinese Cabbage

    • 摘要: 长链非编码RNA(lncRNAs)是一种长度至少为200个核苷酸且缺乏显著编码能力的功能性非编码RNA分子,在植物的生长发育和胁迫应对过程中发挥重要调控作用。大白菜(Brassica rapa L. ssp. pekinensis)属于芸薹属蔬菜作物,是我国种植面积最大的蔬菜作物之一。大白菜杂交种具有明显的杂种优势,杂合状态下基因表达调控网络发生改变,激发新的调控机制,使得基因表达水平发生波动,引起各种生理生化反应的变化,进而导致性状改变、形成优势表型。lncRNAs作为非编码调控RNA,可以通过顺式作用和反式作用直接调控基因表达,或作为miRNAs前体或者靶标间接调控基因表达,导致基因差异性表达。因此,lncRNAs可能在大白菜杂种优势的形成中起重要调控作用。随着高通量测序技术的不断发展和芸薹属作物相关参考基因组数据的发表,大白菜lncRNAs的种类、数量和功能被不断探索。研究发现,大白菜中存在5种类型的lncRNAs:基因间、内含子、正义、反义和双向lncRNAs,其中基因间lncRNAs的数量最多。lncRNAs可调控大白菜的春化和花粉发育过程,还可调控大白菜应对生物和非生物胁迫,如霜霉病菌、尖孢镰刀菌和高温等。该文总结了植物lncRNAs的来源、分类和调控蛋白质编码基因表达的方式,以及大白菜中鉴定到的lncRNAs种类、数量及其生物学功能,为研究lncRNAs在大白菜的生长发育和遗传调控中的生物学功能提供重要参考。

       

      Abstract: Long non-coding RNAs (lncRNAs) are functional non-coding RNA molecules that are at least 200 nucleotides in length and lack significant coding ability, having important regulation effects on plant growth and development and response to stress. Chinese cabbage (Brassica rapa L. ssp. pekinensis) belongs to the Brassica vegetable crop and is one of the vegetable crops with the largest planting area in China. Chinese cabbage hybrids exhibit significant hybrid vigor. In the hybrid state, the gene expression regulatory network undergoes alterations that stimulate new regulatory mechanisms, resulting in variations in gene expression levels. These changes subsequently affect various physiological and biochemical reactions, ultimately leading to modifications in traits and the emergence of a dominant phenotype. As a type of non-coding regulatory RNA, lncRNAs can directly regulate gene expression through both cis-acting and trans-acting mechanisms, or indirectly regulate gene expression as either precursors or targets of miRNAs, which contributes to differential gene expression. Consequently, lncRNAs may play a crucial regulatory role in the heterosis formation of Chinese cabbage hybrids. With the continuous development of highthroughput sequencing technology and the publication of reference genome data related to Brassica, the types, number and functions of Chinese cabbage lncRNAs have been continuously explored. The studies find that there are five types of lncRNAs in Chinese cabbage: intergenic lncRNAs, intron lncRNAs, sense lncRNAs, antisense lncRNAs and bidirectional lncRNAs, of which the number of intergenic lncRNAs is the largest. lncRNAs can regulate the vernalization and pollen development in Chinese cabbage. In addition, lncRNAs can also regulate Chinese cabbage's response to biotic and abiotic stresses, such as downy mildew, fusarium oxysporum, and high temperature. This paper summarizes the source, classification and ways that regulate the expression of protein-encoding genes in plant lncRNAs, as well as the types, number and biological functions of lncRNAs identified in Chinese cabbage, providing important references for studying the biological functions of lncRNAs in regulating the growth, development and genetic regulation of Chinese cabbage.

       

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