Abstract:
Objective The study was carried out to identify the SmTCP gene and analyze the members of SmTCP transcription factor family in eggplant. The determination of its expression under stress and hormone treatment laid a foundation for further study of the function of SmTCP gene in eggplant.
Method The members of SmTCP gene family were identified from the eggplant genome. The physicochemical properties and distribution of members of SmTCP transcription factor family in eggplant were analyzed by bioinformatics methods. Eggplant seedlings were treated with high temperature (42 ℃), low temperature (4℃), salt stress (200 μmol/L NaCl solution), heavy metal (100 μmol/L CdCl2 solution), Pseudomonas solanacearum, salicylic acid and abscisic acid. The expression of SmTCP gene in eggplant under seven treatments was analyzed by qRT-PCR.
Result A total of 32 SmTCP members were identified and analyzed by bioinformatics method. The results of qRT-PCR analysis showed that the SmTCP gene of eggplant was up-regulated in response to biological and environmental stress. Under hormone treatment, the SmTCP of eggplant was up-regulated generally after ABA treatment, and the SmTCP of eggplant was down-regulated generally after SA treatment. The contents of SmTCP in leaves and fruits were higher.
Conclusion It was found that the SmTCP gene of eggplant express in response to stress. The expression of different SmTCP members in response to stress and the expression levels in various organs are different. Most of the Class I TCP members of eggplant are significantly up-regulated under heat stress. Therefore, it is speculated that the SmTCP gene of eggplant may be involved in resisting adversity stress.
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