Abstract: With the advent of CRISPR technologies, almost at any specific locus in any genome of animal or plant, large-size DNA fragment can be inserted or replaced seamlessly following the occurrence of CRISPR nuclease-produced double-strand break (DSB) and donor DNA, and the induction of homology-directed repair (HDR) can be realized. At present, the homologous recombination (HR)-based CRISPR precision gene editing plays increasingly important roles in crop gene functional analysis and new techniques breeding. In this review, aiming at effective realization of HR-based CRISPR precision gene editing in plant cells, we briefly review the two HR-based mechanisms underlying CRISPR precision gene editing, i.e., synthesis-dependent strand annealing (SDSA) and non-homologous end joining (NHEJ) assisted single strand annealing (SSA). Based on this, we elaborate the DSB-producing and HDR-inducing CRISPR nucleases and DNA/RNA donors, mainly including Cas9/12 and fusion proteins, sgRNA/crRNA and modifiers, and donor DNA/RNA and modifiers. Furthermore, we summarize the adopted delivery systems of CRISPR components and donor DNA/RNA during plant genetic transformation, in light of keeping spatiotemporal consistency of DSB and donor DNA/RNA to improve HR efficiency. Finally, we provide an outlook for the promising applications in crop functional genomics study and new techniques breeding of HR-based CRISPR precision gene editing.