不同处理方法对百香果种子萌发的影响

    Effects of Different Treatments on Seed Germination of Passion Fruit

    • 摘要:
      目的 研究百香果种子的萌发特性,为百香果种子育苗及杂交后代种子萌发、性状观察奠定基础。
      方法 以百香果种子为试验材料,研究不同水温(25、45、65、85、100 ℃)、浓硫酸浸泡时间(0、0.5、1、2、5、8、10、15 min)、赤霉素浓度(0、200、400、800 mg/L)、培养温度(18、28、35、18~28、28~35 ℃)、有无种壳及不同物理处理方式(针刺、磨砂)对百香果种子萌发的影响,以发芽率、发芽势、发芽指数为考查指标,确定种子萌发适宜条件。
      结果 百香果种子在65 ℃温水中自然冷却浸泡24 h发芽率最高(57.78%);浓硫酸最适浸泡时间为8 min,发芽率、发芽势和发芽指数分别为77.38%、77.38% 和3.39;赤霉素浓度为400 mg/L时发芽率高达81.11%;最适发芽培养温度为28~35 ℃变温,发芽率为64.44%;全剥壳的百香果种子发芽率达76.67%;磨砂和针刺处理对种子萌发的影响差异不显著,但针刺不好控制容易伤到种胚,以磨砂为佳,发芽率为60%。
      结论 百香果种子适宜用65 ℃温水自然冷却浸泡24 h或浓硫酸处理8 min后浸泡24 h,采用磨砂处理或400 mg/L赤霉素浸泡24 h可有效提高种子发芽率,适宜的萌发培养温度为28~35 ℃变温。

       

      Abstract:
      Objective The study was performed to explore the germination characteristics of Passiflora edulia Sims seeds in order to lay a foundation for seed seedling raising and seed germination and character observation of hybrid offspring of P. edulia.
      Method P. edulia seeds were used as experimental materials to study the effects of different water temperatures (25, 45, 65, 85, 100 ℃), time of soaking in concentrated sulfuric acid (0, 0.5, 1, 2, 5, 8, 10 and 15 min), gibberellin concentrations (0, 200, 400 and 800 mg/L), culture temperatures (18, 28, 35, 18-28, 28-35 ℃), seed shells and different physical treatment methods (acupuncture and sanding) on seed germination of P. edulia. Taking germination rate, germination potential and germination index as test indexes, different germination conditions were evaluated to determine the suitable ones for seed germination.
      Result The germination rate of P. edulia seeds soaked in 65℃ warm water for 24 h was the highest (57.78%). The optimum soaking time in concentrated sulfuric acid was 8 min, the germination rate, germination potential and germination index were 77.38%, 77.38% and 3.39%, respectively. The germination rate was 81.11% when the soaking concentration of gibberellin was 400 mg/L. The optimum culture temperature for germination was 28-35℃, and the germination rate was 64.44%. The seed germination rate of P. edulia was 76.67%. There was no significant difference in seed germination between physical treatment and acupuncture treatment, but acupuncture was not well controlled and easy to hurt seed embryo. Grinding treatment was the best, and the germination rate was 60%.
      Conclusion The seeds of P. edulia are suitable to be soaked in 65℃ warm water for 24 h or soaked in concentrated sulfuric acid for 8 min for 24 h; The seed germination rate can be effectively improved by grinding treatment or soaking in 400 mg/L gibberellin for 24 h. The suitable germination culture temperature is 28-35℃.

       

    /

    返回文章
    返回