Abstract:
Objective Combining with the seawater environmental conditions in Fangchenggang sea area along the coast of Guangxi, the main physical and chemical factors of the growth of Chaetoceros muelleri was explored and the optimal growth environment conditions were screened out in order to provide technical data for large-scale production.
Method The effects of salinity, temperature and light on the growth of C. muelleri were studied by single factor method. The experimental design was as follows: seven gradients of 0‰, 5 ‰, 10‰, 20‰, 30‰, 35‰ and 40‰ were set for salinity test, four gradients of 25, 28, 31 and 34 ℃ were set for temperature test, and three gradients of 1 000, 4 000 and 7 000 lx were set for the light intensity.
Result C. muelleri could grow normally with the salinity of 5‰ - 40‰, among which the salinity of 20‰ was the optimum growth condition. Under the condition of high temperature culture, the cell concentration of C. muelleri reached the maximum at 31 ℃. The lower the temperature, the slower the growth rate, the longer the stable period; the higher the temperature, the faster the growth rate and the shorter the stable period. 4 000 lx was the most suitable light intensity for the growth of C. muelleri. The higher the light intensity, the longer the stability period.
Conclusion The best growth conditions for C.muelleri in Fangchenggang sea area along the coast of Guangxi were: 20 ‰ salinity, 31 ℃ temperature and 4 000 lx light intensity, Under such conditions, both the growth rate and algal cell density reached the highest values. Excessive or low salinity, temperature and light could inhibit the growth of C. muelleri.
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