Abstract: Chinese herbal medicine is the material basis for the sustainable development of the traditional Chinese medicine industry and is of great significance for promoting the comprehensive development of the society and economy. Excellent seedlings are the bases for the standardized production and efficient planting of Chinese herbal medicines, and are also important parts of Chinese medicine agriculture. In the production of Chinese herbal medicine agriculture, the infection of pathogenic bacteria or viruses is one of the main reasons for the decrease in the yield and quality of Chinese herbal medicines. In particular, asexual propagation of traditional Chinese medicine varieties is subjected to repeated infection by various bacteria or viruses for a long time in the natural environment, which affects the photosynthetic efficiency and leads to weak growth potential of seedlings, degradation of varieties, and reduced planting efficiency. The seedlings cultivated with plant detoxification technology can effectively avoid the spread and accumulation of pathogens or viruses, obtain healthy seedlings, and have important application value and commercial prospects. Using plant detoxification technology to cultivate healthy seedlings, to achieve storage and rejuvenation, and promote the development of traditional Chinese medicine seed industry. Plant detoxification technology is a modern biotechnology, mostly based on plant tissue culture technology The use of plant detoxification technology accelerates the promotion of fruit trees and crop varieties, bringing a new technological revolution to agriculture. Using plant detoxification technology to cultivate healthy Chinese herbal medicine seedlings can achieve the rejuvenation of seedlings, enhance the resistance of Chinese herbal medicine seedlings, and promote the development of Chinese herbal medicine seedling industry. This paper reviews the technical methods of plant detoxification and its research progress and application status in Chinese herbal medicine seedlings, and discusses the standardized production of healthy seedlings of Chinese herbal medicines with plant detoxification technology, with a view to providing a reference for the sustainable development of Chinese herbal medicine seed industry.