广薯87鲜薯和蒸薯挥发性风味成分分析

    Analysis of Volatile Flavor Components of Fresh and Steamed Sweet Potato Guangshu 87

    • 摘要:
      目的 研究广薯87在蒸制前后挥发性风味成分的变化情况,为鲜食型甘薯风味物质改良提供参考。
      方法 采用顶空固相微萃取(HS-SPME)和气相色谱-质谱联用仪(GC-MS)技术对广薯87鲜薯及蒸薯的挥发性风味成分进行比较和分析。
      结果 广薯87鲜薯检测出71种挥发性物质,主要包括醛类(40.52%)、萜类(16.18%)、杂环类(15.17%)、醇类(11.59%)、酯类(7.94%)、芳烃类(4.49%)、酮类(1.62%)化合物等;蒸薯检测出69种挥发性物质,主要包括醛类(47.36%)、芳烃类(21.39%)、萜类(10.33%)、卤代烃类(7.07%)、醇类(3.24%)、酮类(2.64%)、酯类(2.61%)化合物等。其中,鲜薯及蒸薯中相对含量最高的挥发物均为苯乙醛,含量分别为16.78%、19.92%,但蒸煮前后相对含量差异不显著。对所检测出的挥发代谢物进行差异筛选,共筛选出47种差异明显的代谢产物。上调的代谢物有14种,下调的代谢物有33种,其中上调倍数最高的化合物为氯乙酸2-苯乙基酯,上调倍数为4.4;下调倍数最高的化合物为2-丙基-1H-咪唑,下调倍数高达13.49。
      结论 结合相对含量及差异分析,广薯87蒸制后相对含量显著上升的主要挥发物成分为2, 4-二叔丁基苯酚和氯乙酸2-苯乙基酯,是最有可能构成广薯87蒸薯特征风味的挥发物。

       

      Abstract:
      Objective The study was carried out to explore the changes of volatile flavor components of Guangshu 87 before and after steaming with a view to providing a basis for the improvement of fresh sweetpotato flavor substance.
      Method The headspace solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) technology were used to compare and analyze the volatile flavor components of fresh and steamed Guangshu 87.
      Result A total of 71 volatile compounds were detected in fresh Guangshu 87, mainly including aldehydes (40.52%), terpenoids (16.18%), heterocycles (15.17%), alcohols (11.59%), esters (7.94%), aromatics (4.49%), ketones (1.62%), and etc. A total of 69 volatile compounds were detected in steamed Guangshu 87, mainly including aldehydes (47.36%), aromatics (21.39%), terpenoids (10.33%), halogenated hydrocarbons (7.07%), alcohols (3.24%), ketones (2.64%), esters (2.61%), and etc.. Among them, the volatile compound with the highest relative content in fresh and steamed sweet potato was phenylacetaldehyde, with the relative content of 16.78% and 19.92%, but there was no significant difference in it before and after steaming. Differential analysis showed that a total of 47 differential metabolites were screened, in which 14 were up-regulated and 33 were down-regulated. The compound with the highest up-regulation factor was chloro-acetic acid, 2-phenylethyl ester with an up-regulation factor of 4.4, and the compound with the highest down-regulation factor was 2-propyl-1H-imidazole, with the down-regulation factor as high as 13.49.
      Conclusion Combining the relative content and differential analysis, the main volatile components with a significant increase in the relative content of Guangshu 87 after steaming are 2, 4-Di-tert-butylphenol and chloro-acetic acid, 2-phenylethyl ester, which are the most likely constituents of the characteristic volatile flavor of steamed Guangshu 87.

       

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