干旱胁迫对毛豹皮樟扦插苗光合作用与叶片组织结构的影响

    Effects of Drought Stress on Photosynthesis and Leaf Tissue Structure of Litsea coreana Levl. var. lanuginosa Cutting Seedlings

    • 摘要:
      目的 了解干旱胁迫对毛豹皮樟扦插苗光合作用与叶片组织结构的影响规律。
      方法 以3年生毛豹皮樟扦插苗为试材,设置4种不同程度干旱胁迫处理:CK(土壤体积持水量为39.6%)、轻度(土壤体积持水量为29.2%)、中度(土壤体积持水量为19.5%)、重度(土壤体积持水量为9.7%),分析毛豹皮樟净光合速率日变化规律、气体交换参数与环境因子相关性及叶片横切组织结构,研究干旱胁迫对其光合作用与叶片组织结构的影响。
      结果 重度干旱下,毛豹皮樟扦插苗“光合午睡”明显,超过2 h,光合日变化平均净光合速率为1.9712 μmol/m2·s,光合能力较低,叶表相对湿度与净光合速率相关系数为-0.462,所以维持叶片正常光合生理需增加空气水分、降低温度;中度干旱下,毛豹皮樟扦插苗叶片叶绿素总量较高、为1.25 mg/L,从而对干旱胁迫作出响应;伴随干旱程度递增,叶片气孔导度降低,中度和重度干旱胁迫下的叶片气孔导度在0.03 mol/m2·s左右,严重影响其水分利用和气体交换,毛豹皮樟扦插苗叶片组织结构也发生变化,上表皮厚度变薄,栅栏组织变厚,海绵组织变薄。
      结论 干旱胁迫能降低毛豹皮樟扦插苗的光合作用,同时也改变叶片组织结构,干旱胁迫下的光合作用能力与叶片组织结构变化规律的相关性需进一步研究。

       

      Abstract:
      Objective The study was carried out to understand the effects of drought stress on photosynthesis and leaf tissue structure of Litsea coreana Levl. var. lanuginosa cutting seedlings.
      Method Three-year-old L. coreana Levl. var. lanuginosa cutting seedlings were used as experimental materials, and 4 drought stress treatments were set: CK (soil volume water holding capacity of 39.6%), mild drought(soil volume water holding capacity of 29.2%), moderate drought(soil volume water holding capacity of 19.5%), and severe drought (soil volume water holding capacity of 9.7%). The effects of drought stress on photosynthesis and leaf tissue structure of cutting seedlings of L. coreana Levl. var. lanuginosa were studied by analyzing the diurnal changes of net photosynthetic rate, the correlation between gas exchange parameters and environmental factors and the leaf transverse tissue structure.
      Result Under severe drought, the "photosynthetic siesta"of Litsea coreana Levl. var. lanuginosa cutting seedlings was obvious, with over 2 hours. The average net photosynthetic rate of photosynthesis was 1.9712 μmol/m2·s, the photosynthetic capacity was low, and the photosynthetic rate correlation coefficient of relative humidity of leaf surface and net photosynthetic rate was -0.462, therefore, it is necessary to increase the air moisture and lower the temperature to maintain the normal photosynthetic physiology of the leaves. Under moderate drought, the total amount of chlorophyll in the cutting seedlings of L. coreana Levl. var. lanuginosa was higher(1.25 mg/L), which could respond to drought stress. With the increase of drought degree, leaf stomatal conductance decreased. Leaf stomatal conductance under moderate and severe drought stress was about 0.03mol/m2·s, which seriously affected its water use and gas exchange. The tissue structure of seedling leaves was also changing, the thickness of the upper epidermis became thinner, the palisade tissue became thicker, and the spongy tissue became thinner.
      Conclusion Drought stress can reduce the photosynthesis of L. coreana Levl. var. lanuginosa cutting seedlings, and it also changes the leaf tissue structure. The correlation between photosynthesis capacity and leaf tissue structure changes under drought stress needs to be further studied.

       

    /

    返回文章
    返回