Abstract: Biological control is an important means used in integrated pest management. As traditional biological control products, natural enemy insects and predatory mites play an irreplaceable role in controlling crop pests and harmful mites and ensuring crop yield and quality. The progresses and achievements in mass propagation, field application and demonstration and promotion of Trichogramma, Anastatus sp. and predatory mites of Guangdong Academy of Agricultural Sciences（GAAS）since 1960s were summarized. And the current situation and future development trends of biological control researches based on natural enemies were explored. Major achievements include: creation of automatic machine for the mass production of artificial host eggs, solving the contamination in continuous passage; first artificial propagation of Trichogramma with Philosamia cynthia ricini, for controlling sugarcane borers, which provided valuable references for propagating Trichogramma with Antheraea pernyi eggs in China; first trial to release Trichogramma by drones in China; improvement and perfection of mass artificial propagation and field application of Anastatus sp., improving the control effect of A. sp. against Tessaratoma papillosa Drury and reducing the application cost; importing the predatory mite, Neoseiulus cucumeris Oudermans, from the Netherlands for the successful control of spider mites and thrips on horticultural crops. These achievements made great contributions to the R&D and application of biological control techniques for controlling crop pests in South China.