Abstract: 【Objective】In order to investigate the effect of glufosinate on the soil animals, Eisenia fetida was selected as tested animal and the comet assay was used to detect DNA damage of E. fetida coelomocytes under the stress of glufosinate with different concentrations.【Method】Under laboratory conditions, the concentrations of glufosinate were set at 0, 37.5, 75, 150 and 300mg/L, and stress time as 24h. The degree of DNA damage was expressed by five parameters, i.e. HDNA content, TDNA content, tail length, tail moment and Olive tail moment.【Result】There were significant differences in five parameters between four treatment groups and the control group. With the increase of glufosinate concentration, TDNA content, tail length, tail moment and Olive tail moment increased, while HDNA content decreased. DNA damage of coelomocytes was caused by glufosinate, and the higher the concentration of glufosinate was, the more serious the DNA damage was. Then, the concentration of glufosinate was reduced stress time extended to 96h, and the same results were obtained.【Conclusion】Glufosinate caused DNA damage to coelomocytes of E. fetida. There was a significant dose-effect relationship between the concentration of glufosinate and the DNA damage of E. fetida coelomocytes.