Abstract: 【Objective】 The study was conducted to optimize oil palm kernel separator and increase the yield of palm oil from press. 【Method】 Taking the separator for kernel of oil palm fruit as the research object, the effects of working time, handling capacity and rotation speed of cutter shaft on the separation rate of pulp of oil palm fruit were analyzed. The pulp was extracted based on an optimal scheme, and a comparison test was conducted between the pure pulp pressing and the mixed pressing of kernel and pulp.【Result】It was found that the working time, handling capacity and rotation speed of cutter shaft had different impacts on the separation rate of oil palm pulp, and the range values were 1.64, 2.45 and 1.15, respectively, indicating that handling capacity had the greatest influence, followed by working time, and then rotation speed of cutter shaft. Based on the analysis of the test data, it was concluded that the separation effect was the best when the working time, handing capacity and cutter shaft speed were 8min, 21 kg and 250 r/min, respectively. By conducting the pressing test to extract oil, the oil yield was 22%-27% when the kernel and pulp were pressed together, and the oil yield was 35%-40% when the pure pulp was pressed.【Conclusion】Reasonable combination of working time, handling capacity and rotation speed of cutter shaft can help to improve the separation rate of pulp of oil palm fruit, and the oil yield of pure pulp is significantly improved after separation .