Abstract: 【Objective】The study was conducted to predict protein structure and features of RAV/PLC in Populus trichocarpa based on bioinformatics analysis.【Method】RAV/PLC gene sequence was obtained from Phytozome database with PLC (Phospholipases C) as the key word and multiple bioinformatics tools were used to analyze RAV/PLC gene and protein sequence.【Results】A 1 650 bp open reading frame (Potri. 018G109200) was identified from Phytozome in P. trichocarpa, encoding 549 amino acids and a special peptide with a RAV domain and a PLC domain. The predicted molecular weight for RAV/PLC was 62.72338 ku and the theoretical pI was 9.16. A higher expression of RAV/PLC was observed in stem compared to leaf, bud and root. An AP2 domain, a B3 domain and a PLC-X domain were contained in RAV/PLC, and there might be a transmembrane helices structure between the AP2/B3 domain and the PLC-X domain. Promoter analysis showed that RAV/PLC might mainly be linked to light and hormone response. String delineated the function network between RAV/PLC and MYB103, WRKY49, ABI5, DXS2; KEGG analysis showed that this gene was mainly related to the RAV transcription factor signaling pathway. And the complete 3D structure was generated via homology modeling.【Conclusion】The information obtained for RAV/ PLC will provide a foundation for the cloning and signal pathway analysis of RAV/PLC.