Abstract: 【Objective】 Ardisia gigantifolia is an important traditional Chinese herbal medicine, which has relied on harvesting from nature for a long time, resulting that resources are almost exhausted. Therefore, it is urgent to domesticate and plant such medicine. In this study, stems of A. gigantifolia were took as the explants to establish a rapid propagation method by tissue culture in vitro.【Method】 The explants were sterilized with 70% alcohol and 0.1% mercury chloride solution for different treatment time. The obtained aseptic explant were inoculated in the modified MS medium containing 6-BA, KT and IBA for cluster buds proliferation. The 1/2 modified MS medium(as basal medium) with NAA and IBA were used to induce rooting for the adventitious buds. After seedling refining, the regenerated plants were transplanted. 【Result】 The result showed that disinfecting the stems via 70% alcohol treatment for 30 seconds with 0.1% mercuric chloride solution treatment for 7-9 mins was the best method. The modified MS medium with 1.0 mg/L 6-BA KT and 0.05 mg/L IBA was the optimal combination for inducing and reproduction. The 1/2 MS medium with 0.2 mg/L NAA and 0.3 mg/L IBA was suitable for rooting. 【Conclusion】 By optimizing the sterilization method, proliferation and rooting medium of explants in rapid propagation system, the method for in vitro culture and rapid propagation of A. gigantifolia was established, which provided seedling foundation for artificial planting.