Abstract: 【Objective】In order to explore the application effect of compound biofertilizer on wheat, this study selected "plateau 338" wheat as test material, and evaluated its application efficiency through field test.【Method】Four experimental groups were set up in the experiment, which were applied with compound biofertilizer, compound fertilizer, substrate only and no fertilization, respectively. The plant height, dry matter weight and yield per hectare at harvest time in the four groups were measured at elongation stage and grain filling stage, and the differences of various indexes between different experimental groups were compared.【Result】The results showed that the dry matter weight of the wheat treated with compound biofertilizer increased by 7.1%~7.5% and 1.0%~10.8% respectively in the elongation and grain filling stages compared with that of the conventional compound fertilizer group, by 3.1%~5.3% and 2.5%~4.5% respectively compared with that of the substrate group, and by 20.3%~29.2% and 19.7%~25.8% respectively compared with that of the control (no fertilization) group. On the yield per hectare of the wheat, compared with the blank control group, the yield increased by 67.4%~75.6%; compared with the conventional compound fertilizer group, the yield increased by 12.4%~13.2%; compared with the substrate group, the yield increased by 8.9%~9.4%, and the differences were significant.【Conclusion】In conclusion, the application of compound biofertilizer can significantly promote the growth of wheat, improve the yield per hectare of wheat, and increase the economic benefits of wheat planting. This study can provide certain preliminary basis and theoretical basis for the large-scale promotion and application of compound biofertilizer in wheat.