Abstract: 【Objective】The experiment was conducted to investigate the effects of recombinant human lactoferrin (rHL) on the growth performance and serum biochemical index of weaned piglets.【Method】One hundred and eighty 28-dayold weaned piglets (with uniform weight), were assigned randomly to 6 treatment groups and there were 3 replicates in each group with 10 piglets per replicate (5 males and 5 females). The control group A were fed with a basal diet, and group B piglets were fed a basal diet supplemented with antibiotic (1 g/kg 10% bacitracin zinc + 200 mg/kg 10% colistin). Groups C, D, E and F were fed with basal diets supplemented with 160, 320, 480 and 640 mg/kg rHL, respectively. The experiment lasted for 28 days.【Result】The results showed as follows: (1) the final weight and average daily gain(ADG) of groups B, C, D, E and F were higher than those of group A (P ＜ 0.05). The daily intake of groups B and E was significantly higher than that of group A(P ＜ 0.05). The ratio of weight gain to intake of groups B, D, E and F was significantly lower than that of groups A(P ＜ 0.05). The diarrhea index of groups B, D, E and F was lower than that of groups A and C(P ＜ 0.05). (2) The concentrations of serum albumin and TP of group E were higher than that those of group A(P ＜ 0.05). The urea nitrogen of groups B and E was higher than that of group A(P ＜ 0.05). (3)The T-AOC level of groups C, D, E and F was higher than that of group A(P ＜ 0.05). The GSH-Px activity of groups D and F was higher than that of group A(P ＜ 0.05). The T-SOD level of group E was higher than that of groups A and B(P＜ 0.05). The MDA content of group A was lower than that of groups C, D, E and F(P＜ 0.05).【Conclusion】 The above data showed the supplemented rHL could improve the growth performance, serum biochemical index and antioxidant ability of weaned piglets. The optimum level of rHL was 480 mg/kg based on the test results.