Abstract: 【Objective】The study was to breed a new cherry tomato variety with high quality and multi-resistance. 【Method】The new cherry tomato hybrid combination was bred by using heterosis theory. The female parent “001F7- 1-1-1-1”is limited growth type, with high fruit setting rate, round fruit shape, green shoulder, single fruit weights about 14.0 g, pink fruit, sweet and sour taste, high combining ability, moderate resistance to bacterial wilt and heat resistance. And the male parent “yellow material 005” is a good comprehensive trait material with resistance to bacterial wilt and disease TYLCV.【Result】The new combination “Yuekeda 301” has strong growth potential and two-pinnate compound leaf. The fruit type index is 0.94 and the fruit shape is round. The fruit has smooth surface, green shoulders (immature fruit), pink colour (mature fruit), long and straight sepals, few dehiscent fruit, with single fruit weight about 17.3 g. The annual yield of greenhouse is 5 000-7 000 kg per 667 m2, and the annual yield of field is 3 000-5 000 kg per 667 m2, which is 24.49%- 37.18% higher than that of the control variety Red Moon. The soluble solids content is more than 8.1% with moderate acid and sweetness. It has resistance loci Ty1, Ty3 of TYLCV and BW12 of bacterial wilt. The incidence of bacterial wilt in the field is 23.2%, and the incidence index of viral disease is 24.5.【Conclusion】The new hybrid combination cherry tomato “Yuekeda 301” cultivar with high quality, high yield and multi-resistance, is suitable for cultivation in protected and open fields in southern China, such as Guangdong.