Abstract: 【Objective】The effect of microbial fertilizer on the yield increase of pumpkin in latosol of Leizhou Peninsula was studied.【Methods】The western small pumpkins were used as test crops. With artificial application of Baogen 120 microbial fertilizer as base fertilizer, the fertigation facilities were used to increase the application of Genduowang 86 microbial agents by drip irrigation, and other base fertilizers and top dressings were routinely managed according to the production of western small pumpkins. The effects of the application of Genduowang 86 microbial agents and Baogen 120 microbial fertilizer on the agronomic traits and economic traits of western small pumpkins planted in latosol of Leizhou Peninsula were determined.【Results】When planting western small pumpkins on the sticky brick soil in Leizhou Peninsula, the Genduowang 86 microbial agents were applied twice by fertigation drip irrigation facilities according to the amount of 10 kg/667m2, and the average yield was 1 802.30 kg/667m2, which was 239 kg higher than that of the control, with a yield increase rate of 15.29%. When planting western small pumpkins on the sand brick soil in Leizhou Peninsula, the base fertilizer was applied with Baogen 120 microbial fertilizer and then the Genduowang 86 microbial agents were applied twice by fertigation drip irrigation facilities according to the amount of 10 kg/667m2, or only the Genduowang 86 microbial agents were applied twice by fertigation drip irrigation facilities based on the amount of 10 kg/667m2, the average yields were 1 026.80 kg/667m2 and 1 022.30 kg/667m2, with increased yields of 140.73 kg and 136.23 kg compared with those of the control, and yield increase rates of 15.88% and 15.37%, respectively. The number of pumpkins per plant and the increase in pumpkin weight lead to the yield increase per unit area.【Conclusion】The application of microbial fertilizer in latosol of Leizhou Peninsula has obvious effect on the yield increase of western small pumpkin. The technology is worth being promoted in the production of western small pumpkin in Leizhou Peninsula.