Abstract: 【Objective】This paper provides a DNA extraction technique based on 96-orifice plates for rice roots, which can significantly improve DNA extraction efficiency. 【Method】 A germinated seed was placed in each orifice of a 96 deep-orifice plate, and a 96-orifice PCR plate was placed under the deep orifice plate to promote the rice root stretching into the PCR plate at an appropriate light and temperature. When the rice seedlings developed with two leaves, the PCR plate was moved to a mixed liquid of dry ice and 95% ethanol, and the roots were captured by freezing treatment. DNA was extracted in bulk by using the modified TRIS-EDTA method and the concentration and purity of DNA was detected . 【Result】 The results showed that all the processes from the seeding to the obtaining of root tissue samples were carried out in 96-orifice plates, which achieved the mass abtainment of the sample tissues and the accurate correspondence of the target individuals. Compared with other methods, the efficiency of extracting DNA from 96 rice samples by the modified TRIS-EDTA method was significantly higher than that of the traditional CTAB method. 【Conclusion】 The batch obtainment of rice root tissues based on 96-orifice plate and DNA efficient extraction method reported in this paper has practical significance for improving of rice molecular breeding efficiency.