Abstract: 【Objective】 The study was to develope a rapid detection method for veterinary drug residues in whole egg, albumen, and yolk. 【Method】 Taking sulfamethazine as a case, a method was developed for rapid detection of sulfamethazine in whole eggs, yolks, and albumens. The sample pretreatment was conducted by using liquid-liquid extraction to achieve the extraction and purification of target compounds. And the target compounds were detected by high performance liquid chromatography-electrospray ionization tandem mass spectrometry that with good selectivity and high sensitivity. 【Result】 As verified by spike matrix test, the method was proved to be stable and reliable. The recoveries of sulfamethazine in whole eggs, yolks, and albumens were in range of 78.4%-85.2%, 77.9%-89.0% and 83.0% - 88.1%, respectively. The detection limit of the method was 0.5 ng/g for the target compounds in whole eggs, yolks, and albumens. Finally, the developed method was applied in sulfamethazine analysis of 15 egg samples from Guangzhou City. The results showed that sulfamethazine was detected in only one sample, and the contents of sulfamethazine in whole eggs, yolks, and albumens were 0.66, 0.94, and 0.47 ng/g, respectively. 【Conclusion】 Due to no column chromatography procedure in sample pre-treatment, the method has the advantages of simple operation, low cost and high sensitivity. In addition, this method has good recovery and stability, which is suitable for rapid detection of sulfadimidine in large quantities of whole egg, albumen, and yolk samples.