Abstract: 【Objective】 The study was to screen cucumber rootstock varieties that suitable for the soil and climatic condition in Sanya City. 【Method】 Cucumber cultivar Jinlv No. 18 was grafted onto 14 widely-applied and independent breeding pumpkin rootstocks, and the effects of grafting on the growth, fruit yield and wax powder of cucumber were studied. 【Result】The highest and lowest survivals of the grafting were 99.14% (Huimei No.1) and 87.57% (Biju F1). The varieties with a survival rate of more than 95% include: Huofenghuang, Weili No. 1, Huangchenggen No. 2, Huimei No. 1, Beinongliangzhen, S84 and S31×S73. The pre-growth advantages of Huofenghuang, Zhuangshi, Beinongliangzhen and S31×S73 were more obvious than those of other treatments; Huofenghuang and Beinongliangzhen had less effect on the thorn and Huofenghuang, Jiatengqingmu, Dongfangzhengdaxiaozi, Beinongliangzhen did not have wax powder and increased the fruit brightness. The plot yield of Beinongzhenmu No. 2 and Huofenghuang increased by 21.54% and 20.54% respectively compared with that of Jinlv No. 18 (CK). 【Conclusion】 After being grafted with Huofenghuang, the cucumber cultivar Jinlv No. 18 obtained high survival rate and vigorous growth and produced fruits with shiny skin and better appearance, with the production increased by 20.54%. Huofenghuang is good rootstock suitable for cucumber production in Sanya.