Abstract: 【Objective】 The study was conducted to explore the enrichment characteristics and extraction changes of Pteris vittata in vivo with the single and interactive treatment of As, Sb and Cd under hydroponiculture conditions. 【Method】 The heavy metal tolerant plant P. vittata was selected as the carrier, and it was cultivated in hydroponics for 7 days with the improved-hoagland nutrient solution. The enrichment characteristics of As, Sb and Cd in different concentrations under adversity stress was explored. And the morphological characteristics of As, Sb and Cd in P. vittata under different treatment conditions were studied when the concentration of 50 mg/L. 【Result】Under single heavy metal adversity stress, the maximum enrichment concentrations of As, Sb and Cd in the underground parts of the P. vittata were 337.56, 7 020.9 , 2 742.58 mg/kg, and the maximum enrichment concentrations in the aboveground parts were 886.6 , 3 058.6, and 4 045.8 mg/ kg, respectively. Only the transfer coefficient of the P. vittata to As is stably larger than 1. At the concentration of 50 mg/L and under the single stress or polymetallic interactive stress, the concentration of As in ethanol and hydrochloric acid in P. vittata was 69.49%-94.25%, the concentration of Sb residue was 47.48%-78.36%, and the concentration of Cd in hydrochloric acid state was 93.67%-98.46%. When Sb was added, the adsorption concentrations of the underground and aboveground parts of the P. vittata to As were increased by 60.83 mg/kg and 144.40 mg/kg, respectively. The addition of As and Cd significantly reduced the concentration of Sb in the roots, stems and leaves of the P. vittata by 72.9%-97.0%. The addition of As and Sb increased the concentration of Cd hydrochloric acid in the roots of the P. vittata by 38.2%-206.5%. 【Conclusion】P. vittata has a strong enrichment ability for Sb and Cd, but only a high enrichment effect. Under the adversity stress concentration of 50 mg/L, As in the P. vittata is mainly in a state of ethanol extraction and hydrochloric acid extraction, Sb is mainly in a state of residue form, Cd in a state of hydrochloric acid extraction condition. Sb can improve the enrichment ability of P. vittata to As. As and Cd are antagonistic to the toxicity of Sb, and As and Sb can improve the Cd in hydrochloric acid state of the P. vittata roots. P. vittata can better restore the soil polluted by As and Sb.