Abstract: 【Objective】Phosphorus is one of the important factors limiting the productivity of Moringa oleifera Lam. In southern China, where there is severe phosphorus deficiency in acid soil, the application of phosphorus-solubilizing bacteria can improve the effectiveness of insoluble phosphorus in soil, improve plant growth and reduce environmental pollution. 【Method】The rhizosphere soil of M. oleifera Lam was chosen as the research material, and the phosphorussolubilizing bacteria was isolated, then screened by of clarity circle method and determined by molybdenum anti-colorimetric method. The strains with phosphorus dissolving ability were preliminarily isolated, and the dominant phosphorus-solubilizing bacteria was further identified by colony morphological characteristics and 16S rDNA high-throughput sequencing. 【Result】 19 strains with organophosphorus dissolving ability and 10 strains with inorganic phosphorus dissolving ability were identified. The maximum phosphorus solubilizing content of lecithin and calcium phosphate by organic and inorganic phosphorus-solubilizing bacteria strains were 26.95 mg/L and 13.48 mg/L, respectively. The amount of phosphorussolubilizing bacteria was not significantly negatively correlated with pH. Two dominant organophosphorus-dissolving strains and two inorganic phosphorus dissolving strains were identified as Bacillus megaterium, Bacillus cereus, Bacillus subtilis and Pseudomonas, respectively. In addition, the result of 16S rDNA high-throughput sequencing showed that the microbial phosphate-dissolving bacteria isolated from the rhizosphere soil of M. oleifera Lam were mainly Paenibacillus, Cupriavidus, Bacillus, Microbacterium, Rhizobium, Mesorhizobium, etc. among which the abundance of Bacillus was the highest.【Conclusion】 The dominant phosphorus-solubilizing bacteria isolated and screened can be used for the development and application of phosphorus-solubilizing bacteria fertilizer, which provides strain resources for improving the root system environment and increasing the productivity of Moringa oleifera Lam.