Abstract: 【Objective】In order to obtain more resources of insect-controlling fungi, the pathogenicity of an Aspergillus strain isolated from the dead larvae of Heortiavites soides in the field was determined and cultured to provide a theoretical basis for the cultivation of highly pathogenic biocontrol fungi.【Method】Aspergillus sp. Q527 was used as experimental material to determine the infection effect of Aspergillus sp. Q527 on H. soides by impregnation method. The culture conditions and sporulation characteristics of Aspergillus sp. Q527 were studied by mycelium growth rate method and sporulation quantity method.【Result】The results showed that the Aspergillus sp. Q527 had a good infection effect on the larvae of H. soides. The corrected infection rate was 83.3% after inoculation for 7 days, and the optimal medium for the growth of Aspergillus sp. Q527 was PDA medium. The average colony cultured on PDA medium was 78.18mm; the pH 8 was suitable for growth and sporulation, with the size of 78.12mm and sporulation amount of 2.61×109; It could not resist high temperature and stopped growing and sporing germination at 65 C. Lactose, as a carbon source, effectively promoted the growth of Aspergillus sp. Q527 mycelium, with the size of 37.97mm; soluble starch, as a carbon source, effectively promoted Aspergillus sp. Q527 sporulation, with the sporulation amount of 2.03×109; peptone, as a nitrogen source, effectively promoted the growth and sporulation of Aspergillus sp. Q527 mycelium, with the size of 51.06 mm and the sporulation amount of 2.96×109 respectively; the best growth condition was in dark state with the colony of 78.17mm and the sporulation amount of 2.63×109.【Conclusion】Aspergillus nomius Q527 is lethal, fast-growing and adaptable to H. soides, and has potential application value in biological control of H. soides.