Abstract: 【Objective】To Screen the biocontrol bacterial strains against bacterial wilt of peanuts. 【Method】 The biocontrol bacterial strains which have antagonistic effects on bacterial wilt of peanuts were screened by plate confrontation method and the bacterial strains with good control effect were screened by pot experiment.【Result】Seven strains with antagonistic effects on bacterial wilt of peanuts were screened from over 300 bacteria strains isolated from rhizosphere soil. Among them, strains Ab, A27 and A38 had a bacterial inhibition diameter of more than 10 mm, and their secretions had an inhibitory effect on the bacteria. The three strains were further subjected to pot experiment, and the results showed that A38 got the best control effect, reaching 52.72%. The strain A38 was identified as Pseudomonas aeruginosa by physiological, biochemical and molecular biological identification.【Conclusion】A biocontrol bacterium with good antagonistic effect against bacterial wilt of peanuts was screened and identified as Pseudomonas aeruginosa.