Abstract: 【Objective】The objective of this paper was to investigate the effect of nitric oxide (NO) on the germination quality of Paris seeds so as to overcome difficulties in seed germination and to provide a basis for the research aiming at promoting Paris germination. 【Method】Seeds of Paris polyphylla var. yunnanensis and Paris polyphylla var. chinensis were selected as the objects of study and treated with 100 μmol/L NO donor-sodium nitroprusside. The seeds treated with water under the same conditions were taken as the control group to record the morphological changes and the germination rates and the electron multiplying CCD ultra-weak light image detection system was used to detect the spontaneous ultra-weak luminescence intensity of the seeds during germination to study the changes of the spontaneous ultra-weak illuminance intensity of the P. polyphylla seeds treated with sodium nitroprusside.【Result】The results showed that the seeds treated with sodium nitroprusside germinated 20 days earlier than the control seeds. There was no significant difference in spontaneous ultra-weak luminescence between the control group and the seeds treated with sodium nitroprusside 0-30 days after germination, and the luminescence intensity of seeds treated with sodium nitroprusside was significantly higher than that of the control group 40-60 days after germination.【Conclusion】Sodium nitroprusside treatment during seed germination of Paris significantly improved the germplasm and promoted germination.