华南地区猪圆环病毒3型分子流行病学研究

Molecular epidemiological investigation of porcine circovirus type 3 in South China

摘要: 猪圆环病毒3型（PCV3）是一种新的猪圆环病毒类型，为了解PCV3在华南地区的分布及其分子流行病学，应用荧光定量PCR方法对华南地区的临床样品进行检测，然后对PCV3阳性样品进行全基因组扩增和测序，将获得的7株PCV3核酸序列与国内外参考毒株进行遗传变异分析。结果表明，华南地区送检的临床样品和猪场的PCV3阳性率分别为46.3%和 69.2%，7株PCV3毒株与国内外的参考毒株同源性为97.4%～99.8%，而ORF2核苷酸及其推导的氨基酸序列同源性分别为96.6%～99.8 %和96.7 %～99.5%。由此可见，PCV3在我国华南地区已经广泛存在。

Abstract: Porcine circovirus 3 （PCV3） is a novel porcine circovirus. However, the distribution and genetic characteristics of emerging PCV3 in south China remains unclear. In this study, we used real-time PCR method to detect PCV3 in clinical samples from south China, then the complete genome sequence of PCV3 was amplified from positive samples and analysis with conference strains. The results showed that the positive rate of PCV3 in individual samples and at the farm level was 46.3% and 69.2%, respectively. The south China PCV3 shared 97.4%-99.8% and 96.6 %-99.8% nucleotide identity in the complete genome and ORF2, respectively. These data suggested that PCV3 is widely distributed in pig populations of South China.