Abstract: In order to study the effects of different tobacco loading methods on neutral aroma components of tobacco leaves，we conducted a comparative experiment between tobacco clamp，loose leaves accumulation and sticking and inserting type. The results showed that there were significant differences in the neutral aroma components in different methods of loading tobacco leaves. Compared with tobacco leaf clamp，the content of carotenoids， aromatic amino acids，browning products，cypress and neopadienes was significantly higher in the middle part of the leaves. However，the content of neutral aroma components was significantly lower in the upper loose leaf tobacco loading method. The evaluation score of neutral aroma constituents was highest in the middle leaf T2 loose leaf accumulation（ 569.24），and highest in the upper leaf T1 smoke clamp（ 495.61）. According to the content of aroma substances content in flue-cured tobacco leaves，the accumulation of loose leaf and smoke clamp can increase respectively the aroma content of the tobacco leaves in the middle and upper parts in Qujing tobacco growing area in Yunnan Province. They could be the most important methods of loading tobacco leaves in research or extension application field.