Abstract: The effects of basic culture medium and organic additives on the germination，differentiation， seedling and rooting of different hybrid seeds of Phalaenopsis amabilis were investigated by vitro culture. The results demonstrated that among four kinds of basic culture medium（ MS，1/2MS，1/3MS，1/4MS ），seed germination in 1/3MS medium was the best，which presenting higher germination rate，larger granules and better growth. At the strongseedling stage，the seedling in 1/2MS medium growed best with sound seedling，high fresh weight and fast leaf differentiation. Among the 3 organic additives（ coconut milk，potato juice and apple juice），the potato juice was the best for seed germination and plant differentiation with 100 g/L concentration and 150 g/L concentration，respectively. The average weight per bottle of different hybrid combinations could reach 26.6 g. At the strongseedling stage，the medium with banana juice and potato juice（ 100 g/L + 150 g/L） was the best，and the seedling presented sound seedling，more root，and higher fresh weight.