锤状中鼓藻培养条件的优化

Optimization of cultural conditions of Bellerochea malleus

摘要: 通过单因子和正交试验，研究了温度、光照、盐度等生态因子及氮、磷、铁、硅、钼、维生素B1、维生素B12 和维生素H 等营养元素对锤状中鼓藻〔Bellerochea malleus（Bright-well）Van Heurek〕生长繁殖的影响。结果表明：锤状中鼓藻生长的适宜温度为15～35℃，最适温度为30℃；适宜光照为1 000～8 000 lx，最适光照为4 000 lx；适宜盐度为5～50，最适盐度为35。筛选出锤状中鼓藻的培养液配方为每升天然海水中加入NaNO3-N 20.0 mg、NaH2PO4-P 1.0 mg、FeSO4-Fe 0.1 mg、Na2SiO3-Si 15.0 mg、Na2MoO4 0.02 mg、VB1 1.0 mg 和VH 0.05 mg。

Abstract: The paper studied effects of temperature，illumination intensity，salinity and nutrients on the growth and reproduction of Bellerochea malleus by one-factor experiment and orthogonal experiments．Results showed that the suitable water temperature was 15-35℃ and the optimal water temperature was 30℃．The suitable and optimal illumination intensity were respectively 1 000-8 000 lx and 4 000 lx．The suitable salinity was 5-50 and the optimal salinity was 35．The optimal prescription for culture solution was as follows：NaNO3-N 20.0 mg/L，NaH2PO4-P 1.0 mg/L，FeSO4-Fe 0.1 mg/L，Na2SiO3-Si 15.0 mg/L，Na2MoO4 0.02 mg/L，VB1 1.0 mg/L and VH 0.05 mg/L.