Abstract: Under the background of“Internet +”，in order to fully stimulate the efficiency and effect of bringing e-commerce into the agricultural industry chain，based on the cooperative game theory，this paper analyzed principal benefit distribution of the agricultural industry chain and constructed a cooperative game model of principal benefit of the agricultural industry chain. Through demonstrating the operability of the model with examples， a satisfactory distribution plan for the benefits of various stakeholders was obtained. The Model avoided the equal distribution of benefits among farmers，processors，sellers and e-commerce platforms，and promoted the stability and development of the agricultural industrial chain under“ Internet + Agriculture.”