Abstract: In this study，the effects of temperature on process of Tremella fuciformis production were investigated. In order to guarantee the quality of mycelium development，according to the effects of various culture temperature（ 20，24 and 28） on physiology and biochemistry variation and enzyme activity variation during mycelial development，the temperature for mycelium growth was determined，respectively，on the basis of T. fuciformis production generated by corresponding mycelium treatment as standard. It was suggested that various temperature treatment exerted substantial effects on T. fuciformis mycelial development and economic characteristics of fruit body. Compared to T. fuciformis compost at 20℃ or 28℃，T. fuciformis compost at 24℃ showed higher secretion level of extracellular matrix degradation enzymes，including carboxymethyl celluloses，amylases，laccases and proteases， which were responsible for substrate degradation. And thus，mycelium cultivated at 24℃ produced more mushroom production with better shape than mycelium grown at 20℃ or 28℃. Therefore，the results demonstrated that the optimal temperature for mycelium growth was 24℃ and that higher or lower temperature conditions than 24℃ exerted detrimental effects on the utilization of substrate by mycelium，resulting in low mushroom production.