玉米籽粒容重QTL 定位

    QTL mapping for test weight of maize kernel

    • 摘要: 以优良玉米自交系农系531(NX531)和航天诱变后的农系531(M-NX531)为亲本组配F2∶3 群体,2012 年在邯郸农科院试验田对亲本和定位群体进行夏播,收获后考种测得籽粒容重性状表型数据,利用SSR 标记构建此群体的遗传图谱,采用复完备区间作图法(ICIM),对玉米籽粒容重性状进行QTL 定位,并对其加性效应和上位性效应进行分析。研究结果表明,构建的遗传图谱总的遗传长度为1 179.8 cM,约占玉米全基因组的85.76%,标记间的平均距离为11.91 cM。通过定位分析,检测到容重的加性QTLs 共3 个,分别位于第1、6、8 条染色体上,共解释20.29% 的表型遗传变异;检测到容重的上位性QTLs 共3 对,涉及6 个位点,分布于第1、3、4、10 共4 条染色体上,并解释67.08% 的表型遗传变异。除了加性效应和显性效应外,上位性效应也是容重的重要遗传基础。

       

      Abstract: In the study,we used the elite inbred line Nongxi531( NX531) and the space mutation inbred line Nongxi531( M-NX531) as the parents to building F2∶3 populations to investigating the bulk density traits,and planted the parents and F2:3 populations in Handan academy of agricultural sciences in the summer of 2012,and measured bulk density traits after harvesting. Using the SSR marker to constructed the genetic map of this population. Used the complete complex interval mapping method( ICIM) for QTL mapping,and evaluated the additive and epistatic effects of QTLs. The results showed that the total length of the genetic map was 1 179.8 cM,which occupied 85.76% of the whole maize genome,and the average distance between the markers was 11.91 cM. We detected 3 additive QTLs through the QTL mapping analysis,which were located on 1st,6th,8th chromosomes,and explained 20.29%phenotypic variation;detected 3 epistatic QTLs,which involving 6 sites,located in 1st,3th,4th and 10th chromosomes,expanded the phenotypic variation in 67.08%. These results indicated that except the additive and dominant effects,epistasis effect was also an important genetic basis for bulk density of maize.