Abstract: Rhamnolipid is an environment-friendly biosurfactant，has widely applied prospect in environmental protection，food，medicine，disease and pest control of plant. The article summarized the application and research progress of rhamnolipid in controlling plant fungicidal disease；And discussed the disease control method of rhamnolipid from hydrolyzing fungal spores，changing cell membrane penetrability，activating plant defense response. The problem and difficulty of rhamnolipid application were also exposed in agriculture. The prospect of rhamnolipid in control of plant fungal disease at last.