Abstract: In order to explore the application availability of truly biodegradable herbicidal mulching film （TBHMF） in winter potato in Guangdong province，three treatments were designed in the field experiment to probe into the effects of different mulching films on yield，quality of potato tuber and soil nutrients in Yuanzhou Town， Boluo County，in 2015. The results showed that the coverage of TBHMF can promote the growth，increase the yield and maintain the nutrients of winter potato tuber，both total tuber yield（ 38 274.6 kg/hm2） and output value（ 146 092.0 Yuan/hm2） were highest，being 16.25% and 15.29% higher than these in the open field. TBHMF has a remarkable herbicidal effect and biodegradability，which will not influence the soil nutrient. TBHMF can entirely replace common mulching film for the coverage of winter potato.