Abstract: In order to known the effect of the corn straw mulching plus nitrogen fertilizer on dry matter accumulation and transportation in wheat‘ Guomai 301’，which was studied by 6 methods of nitrogen application in Xiayi county of Shangqiu city. The results indicated that different nitrogen application methods had remarkable effect on dry matter accumulation and transportation in“ Guomai 301”，and the largest influence on mature stage. Jointing stage，booting stage，flowering period and mature period of dry matter accumulation were showing change trend of unitary quadratic equation with the increasing of application rate of nitrogen，and the nitrogen rate of the highest dry matter of the four fertility periods were 269.12 kg/hm2，277.39 kg/hm2，260.88 kg/hm2，269.77 kg/hm2，respectively. They were the highest of mature dry weight，grain yield，transfer percentage of dry matter and contribute percentage in N3 treatment（ 262.5 kg/hm2 N application）