Abstract: AhNCED1，the rate-limiting enzyme in the ABA biosynthetic pathway was found to be related with peanut drought resistance. The ELISA detection system was optimized to analyze the expression of AhNCED1 protein. The expression of AhNCED1 in leaves of 7 different peanut varieties under drought stress of 24 h was detected by ELISA system. No significant difference was found among different peanut varieties under normal condition. On the contrary，the expression of AhNCED1 proteins of seven peanut varieties remarkably increased under drought stress. The expression change of AhNCED1 of peanut varieties was Zhonghua 16> Puhua 28>Huayu 19>Fuhua 4>Zhonghua 6>Huayu 33>Haihua 1，respectively，which was consistent with the results of QPCR and WB. The ELISA and QPCR for the detecting AhNCED1 expression in different peanut varieties were recommended for selection of droughtresistant peanut varieties rapidly and largely，providing technology support for screening peanut varieties with drought resistant.