Abstract: Tobacco bacterial wilt caused by Ralstonia solanacearum is a widespread and serious disease in China and is difficult to control. Nutrition is the material basis of microbial metabolism and all life activities. Analyzing the effects of nutrition elements on the growth of R. solanacearum is important for inhibiting the growth of pathogenic bacteria through nutrition regulation measures so as to the bacterial wilt disease control. The growth of R. solanacearum under nine kinds of cations，carbon source，nitrogen source and pH were compared in this study. The results showed that R. solanacearum growth under different cations was different. Under the concentration of 1 mmol/L， little influence of Ca2+，K+，Mg2+，Mn2+，Fe2+，Fe3+ were observed on R. solanacearum growth，and their effects were different from Cu2+ and Al3+. Under the conditions of 10 mmol/L of Mn2+，Fe2+，Fe3+，Cu2+ and Al3+ respectively， the R. solanacearum growth was inhibited significantly. When the concentration of Ca2+ and K+ was 30 mmol/L，the R. solanacearum growth was inhibited，while the concentration of Mg2+ was 60 mmol/L，R. solanacearum grew well. In addition，the results also showed that pH value 7，sucrose and peptone were the best for R. solanacearum growth. Nitrate nitrogen source was against R. solanacearum growth.