纤维素降解菌的筛选、鉴定和糖化水平研究

Isolation，identification and saccharification level of cellulose degrading bacteria

摘要: 将前期从木薯生境中分离、保存的298 个菌株，通过平板测试，筛选到57 个可产纤维素酶活的菌株，其中酶活性效果最好的菌株（水解圈最大，将其赋值得分为3）所占比例为40.9%，酶活性依次减弱的菌株所占比例（赋值得分为2 和1）分别占27.9% 和24.6%。采用DNS 法测定57 个菌株的糖化水平和酶活力，结果显示，10 株细菌具有较好潜力降解纤维素，其中菌株HWY-3-9 的糖化和酶活力最好，产糖量为0.1852 mg/mL，酶活力为0.0012 U/mL，经鉴定为解淀粉芽孢杆菌。

Abstract: In this study，298 bacteria were isolated from cassava microenvironments and 57 strains with cellulase activity were gained by in vitro detection test. Results showed that strains with big hydrolysis circle (the score of 3) accounted for 40.9% and strains with weakly hydrolysis circle（the score of 2 and 1） accounted for 27.9% and 24.6%，respectively. The saccharification level and enzyme activity were tested by DNS method，and we found 10 potential strains with efficient ability of degradation of cellulose，in which strain HWY-3-9 had the best saccharification and enzyme activity， the sugar content was 0.1852 mg/mL and enzyme activity was 0.0012 U/mL， and it was identified as Bacillus amyloliquefaciens.