螺旋藻基因组中糖苷酶类基因的序列分析
Current situation and countermeasures of integration and development of leisure agriculture tourism industry in Guangdong province—A case study of Conghua，Guangzhou
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摘要: 在NCBI 基因组数据库中可检索到3 个钝顶螺旋藻藻株、1 个极大螺旋藻藻株、1 个盐泽螺旋藻藻株和1 个螺旋藻属未定种的藻株基因组序列。6 个螺旋藻基因组序列来源的糖苷酶基因共计32 个，编码蛋白含260～1 084 个氨基酸，BLAST 序列比对显示，与非螺旋藻种属来源的已知序列的相似性在49%～84% 之间。系统进化分析表明，32 个糖苷酶基因聚类成8 个簇，另有3 个基因分别单独构成进化分支。酶学功能预测显示，这些糖苷酶基因分别属于8 个糖苷酶家族（GH3、GH9、GH13、GH23、GH25、GH38、GH57、GH104），具有8 种糖苷酶催化活性（纤维素酶、溶菌酶、α- 甘露糖苷酶、α- 淀粉酶、普鲁兰酶、1，4-α- 葡聚糖分支酶、肽聚糖结合功能、β- 葡萄糖苷酶）。Abstract: There are genome sequences of three Arthrospira platensis strains，an Arthrospira maxima strain， a Spirulina subsalsa strain，an Arthrospira sp. strain in the NCBI genome database up to date. 32 glycosidase genes can be retrieved from the 6 Spirulina genome sequences，which have 260-1 084 amino acids in length. By BLAST analysis in GenBank，the similarity with the known sequences from the non-Spirulina species was 49%-84%. Phylogenetic analysis indicated that the 32 glucosidase genes were divided into 8 groups，and the other 3 genes constituted an independent evolutionary branch，respectively. The enzyme function prediction of these glucosidase genes showed that they belonged to 8 glucosidase families: GH3，GH9，GH13，GH23，GH25，GH38，GH57， GH104，and 8 kinds of glycosidase catalytic activity: cellulase，lysozyme，α-mannosidase，α-amylase， pullulanase，1，4-α-glycogen branching enzyme，peptidoglycan binding function，β-glucosidase.
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