杜洛克猪全基因组连锁不平衡分析

Genome-wide linkage disequilibrium analysis in Duroc pigherd

摘要: 利用猪Illumina Porcine SNP60K 芯片对福建某核心种猪场杜洛克猪216 个个体进行基因型检测，基于该高密度SNP 芯片数据，运用Haploview 软件计算全基因组连锁不平衡并构建杜洛克猪连锁不平衡图谱。结果表明，该杜洛克猪群体不同染色体上相邻标记间r2 存在波动，波动范围为0.46～0.59，相邻标记间的平均连锁不平衡程度r2 为0.52，SSC10 的r2 最低（平均为0.46），SSC6 的r2 最高（平均为0.59），连锁不平衡水平随着标记间距的增加而衰减、变异程度随之减小。该研究结果可为杜洛克猪遗传分析及全基因组选择研究提供参考。

Abstract: In this study，we collected 216 Duroc pigs from a breeding herd in Fujian province and genotyped them with Illumina Porcine SNP60K Bead Chip. Based on the high-density SNP data，the genome-wide LD was calculated with Haploview. Results showed that LD reduced as the marker intervals increasing. The r2 between adjacent markers ranged from 0.46 to 0.59 in the Duroc population. The mean r2 was 0.52 among adjacent markers across the genome，with the minimum in SSC10（ r2 =0.46），and the maximum in SSC6（ r2 =0.59）. This study provides useful information for further genome-wide analysis of Duroc pig.