Abstract: Penaeus vannamei is susceptible to blach change，microbial contamination，drying lose during storage，puzzling aquatic product industry. Sodium phytate，nisin and konjak oligosaccharide，as natural ingredients were selected from test of inhibition shrimp head dark，anti-bacteria and water-holding. Then complex formula of water and fresh-keeping for shrimp was developed for preparation with carboxymethyl chitosan，which had better water and fresh-keeping capacity. The results showed that the shelf life of shrimp could prolong for 3-4 days，and drying loss could be reduced by 50% after 6 months frozen storage，with regeant of 0.3% carboxymethyl chitosan，0.05% nisin，0.3% sodium phytate and 0.3% konjak oligosaccharide. Natural biological water and fresh-keeping agent without sulfur and phosphorus，was safe，efficient，had a larger space for development and prospects in current to food quality requirements.