犏牛、牦牛睾丸组织中Ghrelin 与GHSR 基因mRNA 表达水平研究

Expression level of Ghrelin and GHSR mRNA in testis of cattle-yak and yak

摘要: 对犏牛、牦牛睾丸组织中生长素（Ghrelin）与生长激素促分泌素受体（GHSR）基因 mRNA 表达水平进行研究。运用荧光定量PCR 方法，检测Ghrelin、GHSR 基因在犏牛、牦牛睾丸组织中mRNA 的表达水平差异，结果显示：在犏牛、牦牛睾丸组织中，Ghrelin 基因mRNA 相对表达量犏牛显著高于牦牛（分别为0.8177±0.0225、0.4656±0.0222，P ＜ 0.05）；GHSR 基因mRNA 相对表达量也是犏牛显著高于牦牛（分别为0.8622±0.0347、0.4722±0.0761，P ＜ 0.05）。相对于牦牛睾丸组织，犏牛睾丸组织中Ghrelin 与GHSR 基因mRNA 均高表达可能是犏牛雄性不育的原因之一。

Abstract: The expression level of Ghrelin and growth hormone secretagogue receptor（GHSR） gene mRNA tested respectively in testis of cattle-yak and yak by using fluorescence quantitative polymerase chain reaction （PCR）. The results showed that Ghrelin mRNA expression level in cattle-yak testis was significantly higher than that in yak（ 0.8177±0.0225，0.4656±0.0222，P ＜ 0.05）. Similarly，GHSR mRNA expression level in cattleyak testis was significantly higher than that in yak（0.8622±0.0347，0.4722±0.0761，P ＜ 0.05）. These results indicated that both Ghrelin and GHSR mRNA expression level in cattle-yak testis was significantly higher than that in yak，which was likely to be one of the reasons for male infertility of cattle-yak.