桑叶γ- 氨基丁酸提取工艺的响应面优化

Optimization of γ-aminobutyric acid extraction from mulberry leaf by response surface methodology

摘要: 利用逐因子试验和 Box-Behnken 试验，以水为溶剂对桑叶γ- 氨基丁酸（GABA）的萃取工艺进行了条件优化研究。结果表明，最佳提取条件为，桑叶粉∶水=1（g）∶30（mL），提取温度50℃，提取时间2.13 h，此工艺条件下提取率为0.464%，其中影响因素贡献率为提取时间﹥ 料液比﹥ 提取温度。

Abstract: By seriatim-factorial experiment and Box-Behnken test，the extraction conditions of γ-aminobutyric acid（GABA） from mulberry leaf with water were optimized. The results showed that the extraction rate was 0.464% under the following modification conditions: the ratio of mulberry leaf powder to water was 1（g）∶30（mL） and treated at 50℃ for 2.13 h. The order of the factors influencing the result was extraction time﹥ratio of mulberry leaf powder to water﹥temperature.