Abstract: To clear the effects of temperature on the growth of banana adventitious bud and its root activity， taking‘ Brazil’ banana（ Musa paradisiaca） as materials in the artificial climate chamber conditions. The results showed that SOD activity first increased and then decreased. POD activity first elevated and reached the highest in the 15-20 d，and then declined and slowly improved after 45 d. CAT activity first fell and increased after 45 d. At 30℃，with the increase of temperature，root activity gradually increased and the free proline content after 20 d rose and began to drop at the 50th d. MDA content decreased gradually and then increased，was lower at the 20th d and the 45th d. Banana adventitious bud grew best at 30℃. At the 25th day under this condition，the root activity reached the highest（ 11.92 mg /g·h），the contents of free praline（ 58.76 μg/g） and MDA（ 7.15 nmol/g） were low，and vigorous growing plantlets（ height 5.02 cm，leaf numbers 3.64，root numbers 4.63±0.06，root length 4.91 cm） were most suitable for transplanting. At the 45th day under this condition，the root activity reached higher （2.33 mg /g·h），the contents of free praline（ 94.83 μg/g） and MDA（ 9.83 nmol/g） were low，and vigorous growing plantlets（ height 6.15 cm，leaf numbers 4.97，root numbers 6.01，root length 6.06 cm） were suitable for transplanting. The results suggested that 30℃ was most suitable for the growth of banana adventitious bud and the most suitable time for transplanting was to train for 25 days.