Abstract: In order to improve the maize resistance to southern corn rust of temperate germplasm，the resistant genes，which came from germplasm of high SCR-resistant in CIMMYT，were introduced in temperate germplasm.The SCR-resistance of temperate germplasm was directionaly improved by pyramiding SCR-resistant genes. 368 plants of a BC2F2 family，which had the same polymorphism with the SCR-resistant parent by detecting with SSR marker phi048，were used to identify their SCR-resistance by artificial inoculation in field. The results showed that 235 plants were highly resistant or resistant，accounting for 63.9%，114 plants were moderately resistant，accounting for 31.0%，19 plants were susceptible，accounting for 5.1%. The results demonstrated that SSR marker phi048 was effective to select SCR-resistant materials in early stage，which could knock out lots of SCR-susceptible lines therefore reduce plenty of field work. The SCR-resistance of temperate germplasm was effectively improved by directional selection with marker assistant combined to identification with artificial inoculation.