Abstract: To establish a fast and simple method for identifying Guangdong summer soybean varieties（ lines），48 primers showing polymorphic bands were screened from 159 SSR primers covering 20 linkage groups of soybean chromosome，and be used to detected 34 Guangdong summer soybean varieties（ lines）. The data number ranked from fragment size of allele variation were analyzed by the software ID Analysis 1.0 . The average number of alleles by each primer was 3.88，and the average of gene diversity index and polymorphism information content（ PIC） of the 48 SSR loci was 0.50 and 0.45，respectively. These primers could be used to identify the Guangdong summer soybean germplasm.Only using five primers（ Sat_267，Sat_235，Satt277，Sat_351 and Sat_292） could identify all 34 materials. A set of molecular ID was established for Guangdong summer soybean varieties（ lines）.