单倍体育种技术在水稻育种中的应用与展望

    Application and Prospects of Haploid Breeding Technology in Rice Breeding

    • 摘要: 水稻(Oryza sativa L.)是我国最重要的粮食作物之一,在保障国家粮食安全中具有重要的战略意义。近年来,随着全球气候变化加剧、耕地资源减少、农业用水日益紧张以及病虫害等生物胁迫频发,极大增加了育种工作者选育兼具高产、稳产性与广适性水稻品种的难度,也对传统育种模式提出严峻挑战。传统杂交育种方法通常需历经6~10个世代才能实现基因型的纯合稳定,不仅周期漫长,而且过程繁琐、效率低下,难以满足当前农业生产对快速育种的需求。而单倍体育种技术作为一种新兴的现代生物育种手段,通过单倍体诱导、单倍体鉴定和染色体加倍3个核心环节,将传统育种中漫长的基因型纯合过程大幅压缩至1~2代即可完成,从而实现育种效率的显著提升。因此,其在水稻遗传改良与品种选育中展现出广阔应用前景和巨大推广潜力,特别适用于聚合抗逆基因、改良品质性状以及实现精准育种。该文系统梳理了单倍体育种技术的理论基础与操作流程,重点介绍水稻单倍体的诱导途径、技术瓶颈、创新进展及育种实践,并展望未来发展方向,为水稻科研与育种工作者提供系统全面的技术参考,推动我国水稻育种技术体系的现代化升级。

       

      Abstract: Rice (Oryza sativa L.), as one of the major food crops in China, holds significant strategic importance in ensuring national food security. However, factors such as climate change, shrinking arable land resources, water scarcity, and biological stresses have greatly increased the difficulty for breeders to develop high-yield, stable-yield and wide adaptability varieties under harsh environmental conditions and poses severe challenges to conventional breeding models. Traditional cross-breeding methods typically require 6 to 10 generations to achieve homozygous genotype, which is not only time-consuming but also cumbersome and inefficient, failing to meet the current demands for rapid breeding in agricultural production. In contrast, haploid breeding technology, as an emerging modern biotechnological breeding approach, compresses the lengthy process of genotype homogenization in traditional breeding into just 1-2 generations through three core steps: haploid induction, haploid identification, and chromosome doubling. This achieves a qualitative leap in breeding efficiency. As a result, it demonstrates broad application prospects and great potential for dissemination in rice genetic improvement and variety development, particularly suitable for pyramiding multiple stress-resistance genes, optimizing quality traits, and achieving precision breeding objectives. This paper systematically reviews the theoretical foundation and operational procedures of haploid breeding technology, with the focus on induction pathways, existing technical bottlenecks, recent innovations, and practical breeding applications in rice. It also provides an outlook on future development directions, aiming to offer comprehensive technical reference for rice researchers and breeders and to promote the modernization of China's rice breeding technology system.